Thymidine Phosphorylase anticorps

N° du produit ABIN6938686

Cet anticorps anti-Thymidine Phosphorylase est un anticorps Souris Monoclonal détectant Thymidine Phosphorylase dans WB, IP et IHC (f). Adapté pour Humain.

Aperçu rapide pour Thymidine Phosphorylase anticorps (ABIN6938686)

Antigène: Thymidine Phosphorylase (TYMP)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thymidine Phosphorylase est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: P-GF-44C

Détail du produit anti-Thymidine Phosphorylase anticorps

Specificité: Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Réactivité croisée (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HUVEC cells. Breast,Spleen, Bladder, Lung or Kaposi tumors.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Thymidine Phosphorylase

Antigène: Thymidine Phosphorylase (TYMP)

Autre désignation: TYMP

Sujet: ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp,Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker)
Cellular localisation: Cytoplasmic and Nuclear

Poids moléculaire: 55kDa

ID gène: 1890, 180903

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2